After SDS-PAGE, the recovery of CP added the same volume of incomplete FreundAdjuvant emulsified completely, injected rabbits by subcutaneous injection.
PAGE电泳后,将表达的CP蛋白切胶回收,研磨成粉后加入等体积的福氏不完全佐剂,乳化制备成抗原。
3
The depot effect is not the only mechanism as Freund have demonstrated that the excision of the material at the injection site does not suppress the adjuvant effect.