Rat tracheal epithelial cell (RTE) grows well in vitro under the support of 3t3 feedercell layer.
原代rte细胞在3 T3滋养细胞支持下，在体外培养条件下可生长。
2
The effect of feedercell type, growth factors, and embryo developmental stage on establishment of sheep embryonic stem cell was also studied in present experiment.
同时，比较了饲养层细胞类型、生长因子、胚胎发育阶段等因素对绵羊胚胎干细胞分离培养效果的影响。
3
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.