Fix cells - Add16% formaldehyde directly into culture medium to obtain a finalconcentration of 1.5% formaldehyde.
固定细胞-把16%甲醛直接地加入培养基获得一个1.5%甲醛的最后一个浓度。
2
High nitrogen concentration led to overgrowth of cells and overproduction of by-products, therefore, decrease of 1, 3-pd finalconcentration and conversion of glycerol to 1, 3-pd.