Methods: the binding rate of DCPB to plasma protein was determined by equilibriumdialysismethod, and the content of CYP450 was assayed by differential spectrum method.
方法：用分光光度法测定cyp450含量，用平衡透析法测定蛋白结合率。
2
Equilibriumdialysis is a classical method in studying on the interactions of proteins with organic small molecules.