Methods Hybridomacellline excreting monoclonal antibody against total aflatoxins was produced by using B cellhybridoma technique.
方法利用B细胞杂交瘤技术,建立分泌抗总黄曲霉毒素单克隆抗体杂交瘤细胞株。
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Objective: To obtain hybridomacellline which can secrete anti-gonyautoxin2,3 (GTX2,3) monoclonal antibodies(mAb) and analysis the character of the mAb.
To generate the recombinant antibodies, the key point is the cloning and the sequencing of the VH and VL genes from antibody secreting hybridomacellline.