Then, antigen was prepared by conjugation of hapten with carrier protein by carbodiimide (EDPC) method and was identified by UV spectroscopy and SDS-PAGE.
然后通过碳二亚胺法将半抗原与载体蛋白偶联制备人工抗原，采用紫外扫描及SDS - PAGE鉴定。
2
Methods T 2 toxin in pyridine reacted with succinic anhydride in a steam bath and converted to T 2HS. At the presence of EDPC, T 2HS conjugated with BSA, then BSA T 2HS was prepared.