The rough extract of Pinggu mushroom PPO was obtained by ammoniumsulfateprecipitation method.
采用硫酸铵沉淀法得到平菇多酚氧化酶粗提液。
2
The enzyme is crushed in osmotic pressure process, and purified in ammoniumsulfateprecipitation and chromatographic process.
酶经渗透压法破碎、硫酸铵沉淀和柱层析纯化。
3
Conclusion: Purified protein and its amino acids were obtained by defatted ammoniumsulfateprecipitation procedure, and the content of protein was acceptable. This method is easily and more feasible.