Methods Peripheral blood mononuclear cells(PBMC) were collected by using lymphocyte Ficoll and the low-osmotic hemolysis method.
方法用淋巴细胞分层法和低渗溶血法提取PBMC。
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This paper reports the successful isolation of islets of Langerhans from the rat pancreas using a modified collagenase digestion, Ficoll density gradient separation and hand-picking technique.