Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.
方法取大鼠胚胎分离成纤维细胞,分别采用细胞悬液法和胰酶消化植块培养法对其进行培养。
2
F group: the culture of fibroblast.
组:单纯成纤维细胞培养组。
3
Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast (MEF) cells for isolation and culturing of embryonic stem (es) cells.