Here we present the application of confocal spot detection combined with fluorescence fluctuation analysis.
This allowed us to condense several data sets taken on the same protein at the same excitation wavelength, but with different confocal geometries, into a single curve.
A single confocal section of pea nuclei is shown.
In a multicolor confocal setup, optimal alignment is essential to guarantee maximal overlap of all detection volume elements in the different spectral ranges.
Experimental traces used to compare to model simulations were obtained using a confocal spot detection methodology, described in detail previously.
1.1Denoting or using a microscope whose imaging system only collects light from a small spot on the specimen, giving greater resolution.
（使用）共焦显微镜的
Example sentencesExamples
He explains that once researchers learned how to mark the prion proteins, they added them to a culture of nerve cells and then began watching and taking photo images with a confocal microscope.
The boundary could be inspected during the experiment from the transmission image produced by the confocal microscope.
The images were recorded in a confocal microscope.
In a confocal microscope, light passes through a pinhole to eliminate photons from outside the image plane.
The component whose diffusion is to be observed must be tagged with a fluorophore so that it can be imaged in the confocal microscope.
Definition of confocal in US English:
confocal
adjectivekänˈfōkəl
1Having a common focus or foci.
共焦的
confocal ellipses
共焦椭圆。
Example sentencesExamples
A single confocal section of pea nuclei is shown.
In a multicolor confocal setup, optimal alignment is essential to guarantee maximal overlap of all detection volume elements in the different spectral ranges.
This allowed us to condense several data sets taken on the same protein at the same excitation wavelength, but with different confocal geometries, into a single curve.
Experimental traces used to compare to model simulations were obtained using a confocal spot detection methodology, described in detail previously.
Here we present the application of confocal spot detection combined with fluorescence fluctuation analysis.
1.1Denoting or using a microscope whose imaging system only collects light from a small spot on the specimen, giving greater resolution.
（使用）共焦显微镜的
Example sentencesExamples
In a confocal microscope, light passes through a pinhole to eliminate photons from outside the image plane.
He explains that once researchers learned how to mark the prion proteins, they added them to a culture of nerve cells and then began watching and taking photo images with a confocal microscope.
The images were recorded in a confocal microscope.
The boundary could be inspected during the experiment from the transmission image produced by the confocal microscope.
The component whose diffusion is to be observed must be tagged with a fluorophore so that it can be imaged in the confocal microscope.