An end of a DNA double helix at which a few unpaired nucleotides of one strand extend beyond the other.
〔生化〕粘性末端
Example sentencesExamples
This created sticky ends on the dsRNA molecules which permitted efficient ligation.
DNA cyclization is achieved when the sticky ends come close enough together to form a duplex.
This works because the enzyme will seek out the corresponding sticky ends on the target DNA, in essence cleaving or cutting out a certain section of the DNA.
Such linkers could be joined by DNA ligase onto the DNA to be inserted and the resulting molecule cut with a restriction enzyme to generate sticky ends.
Here, we have adapted a method we developed previously for the ligation of DNA triangles; we solve the phosphorylation requirement problem by restricting to create phosphorylated sticky ends.
Definition of sticky end in US English:
sticky end
noun
Biochemistry
An end of a DNA double helix at which a few unpaired nucleotides of one strand extend beyond the other.
〔生化〕粘性末端
Example sentencesExamples
This works because the enzyme will seek out the corresponding sticky ends on the target DNA, in essence cleaving or cutting out a certain section of the DNA.
This created sticky ends on the dsRNA molecules which permitted efficient ligation.
DNA cyclization is achieved when the sticky ends come close enough together to form a duplex.
Here, we have adapted a method we developed previously for the ligation of DNA triangles; we solve the phosphorylation requirement problem by restricting to create phosphorylated sticky ends.
Such linkers could be joined by DNA ligase onto the DNA to be inserted and the resulting molecule cut with a restriction enzyme to generate sticky ends.